Dev115642 1..11
نویسندگان
چکیده
Nonsense-mediated mRNA decay (NMD) represents a highly conserved RNA surveillance mechanism through which mRNA transcripts bearing premature termination codons (PTCs) are selectively degraded to maintain transcriptomic fidelity in the cell. Numerous in vitro studies have demonstrated the importance of the NMD pathway; however, evidence supporting its physiological necessity has only just started to emerge. Here, we report that ablation of Upf2, which encodes a core NMD factor, in murine embryonic Sertoli cells (SCs) leads to severe testicular atrophy and male sterility owing to rapid depletion of both SCs and germ cells during prepubertal testicular development. RNA-Seq and bioinformatic analyses revealed impaired transcriptomic homeostasis in SC-specific Upf2 knockout testes, characterized by an accumulation of PTC-containing transcripts and the transcriptomewide dysregulation of genes encoding splicing factors and key proteins essential for SC fate control. Our data demonstrate an essential role ofUPF2-mediatedNMD in prepubertal SCdevelopment
منابع مشابه
Dev115642 352..362
Nonsense-mediated mRNA decay (NMD) represents a highly conserved RNA surveillance mechanism through which mRNA transcripts bearing premature termination codons (PTCs) are selectively degraded to maintain transcriptomic fidelity in the cell. Numerous in vitro studies have demonstrated the importance of the NMD pathway; however, evidence supporting its physiological necessity has only just starte...
متن کاملLine 806 (06/25/17) -- Metro Rail - Downtown LA - Downtown Santa Monica
— — — 3:26A 3:29A 3:31A 3:35A 3:37A 3:39A 3:41A 3:42A 3:44A 3:48A 3:51A 3:53A 3:55A 3:58A 4:04A 4:06A — — — 3:47 3:50 3:52 3:56 3:58 4:00 4:02 4:03 4:05 4:09 4:12 4:14 4:16 4:19 4:25 4:27 — — — 3:59 4:02 4:04 4:08 4:10 4:12 4:14 4:15 4:17 4:21 4:24 4:26 4:28 4:31 4:37 4:39 — — — 4:11 4:14 4:16 4:20 4:22 4:24 4:26 4:27 4:29 4:33 4:36 4:38 4:40 4:43 4:49 4:51 — — — 4:26 4:29 4:31 4:35 4:37 4:39 4...
متن کاملLine 803 (12/11/16) -- Metro Rail - Metro Green Line - RailEastbound to Norwalk - Westbound to Redondo Beach
— — — — — — — — — — 3:36A 3:42A 3:58A 4:08A — 3:53A 3:55A 3:57A 3:59A 4:02A 4:05A 4:07A 4:09A 4:11A 4:13 4:16 4:21 4:25 — 4:21 4:23 4:25 4:27 4:30 4:33 4:35 4:37 4:39 4:41 4:44 4:49 4:53 4:31A 4:33 4:35 4:37 4:39 4:42 4:45 4:47 4:49 4:51 5:00 5:03 5:08 5:12 4:50 4:52 4:54 4:56 4:58 5:01 5:04 5:06 5:08 5:10 5:12 5:15 5:20 5:24 5:03 5:05 5:07 5:09 5:11 5:14 5:17 5:19 5:21 5:23 5:25 5:28 5:33 5:37...
متن کاملLine 804 (06/26/16) -- Metro Rail - Metro Gold Line (Azusa – Union Station – East LA)
4:20A 4:30A 4:34A 4:40A 4:44A 4:50A 4:56A 4:59A 5:04A 5:05A 5:09A 5:12A 5:17A 5:20A 5:24A 5:28A 5:32A 4:44 4:54 4:58 5:04 5:08 5:14 5:20 5:23 5:28 5:29 5:33 5:36 5:41 5:44 5:48 5:52 5:56 5:04 5:14 5:18 5:24 5:28 5:34 5:40 5:43 5:48 5:49 5:53 5:56 6:01 6:04 6:08 6:12 6:16 5:24 5:34 5:38 5:44 5:48 5:54 6:00 6:03 6:08 6:09 6:13 6:16 6:21 6:24 6:28 6:32 6:36 5:44 5:54 5:58 6:04 6:08 6:14 6:20 6:23 ...
متن کامل11 - Hydroxysteroid dehydrogenase type 1 is a predominant 11 - reductase in the intact perfused rat liver
11 -Hydroxysteroid dehydrogenase type 1 (11 -HSD-1), a regulator of intrahepatocellular glucocorticoid activity, is bidirectional in homogenates but catalyses 11 reduction (regenerating glucocorticoid) in intact primary hepatocytes in culture. To examine this discrepancy at the whole-organ level, we examined 11 -HSD-1 activity in the intact bivascularly perfused rat liver. On a single pass thro...
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تاریخ انتشار 2014